Hi @quantumodo,
Thank you for sharing your save file. We have identified the issue in step 180.
The reason you cannot trigger “08031” (Rapid Response) is the same as I mentioned before. When “31022” (Spider-Man: Otto Octavius) was defeated, you triggered “27023” (Web of Life and Destiny), which caused the deck to shuffle. After the shuffle, “31022” was no longer in your discard pile, so the condition for “08031” was not met.
If you have any further questions or need clarification, please feel free to ask!