Looks good! That's a more detailed list than the broad strokes I was thinking about, but overall it's got what I was getting at. A couple of clarifications:

3a: The 'click the hover icon' mechanic works fine for explaining the more advanced buildings, but I think that the more fundamental ones should be more actively explained (instead of leaving it up to the player to see if they click the icon to learn about the building, or not). I think an explanation in a tip or the quest screen would be better.

4: I don't think you need to go over every need at once here. You could say something like "Your citizens will need energy, food, etc. These can be fulfilled by different buildings." After this, you launch the tip explaining missions, and that leads the player to go through the first four missions.

5: I'd actually remove all the blueprints and then fire off the blueprints tip (and enable the blueprints button) once the player finds their first one.

Kinda as a corollary to point 5, I felt like there was too much "stuff" in general at the beginning of the game. Things like the red rocks (and other rare resources), blueprints, and disabled buildings (no blueprint yet) could probably be hidden from the player entirely. Even if the player can't do anything with them yet, these currently-unusable things divert the player's attention away from learning the base systems. You could keep the tutorial more focused by removing non-essential options until the tutorial is over. (I'd also make the tutorial optional, so that you and other experienced players don't have to go through it every time.)

I remember playing a game that was a similar style some time ago called Kingdom: New Lands. I honestly don't remember if they even had a tutorial, but it could be useful to look at that game and see how it tries to teach you its systems.

I'll give the game another play later this week and let you know if I have more feedback.

Cheers,
Josh

The tutorial work is nice! It seems easier to get into, although I do have knowledge from my first playthrough. Here's some more playtesting notes in no particular order.

Built a stage, but then thought I should move it. I couldn't figure out how to change the placement before it was built, and after the 'garbage can' didn't seem to schedule it for destruction (or if it did, a citizen never got around to it.)

I like the priority system, but I'm a little confused about how priorities interact with professions.
    -- I would add numbers to the priority squares for players that are red-green colorblind.
    -- For other examples of priority systems for jobs, check out Dwarf Fortress and Rimworld.

The UI buttons appearing from right to left (with empty space inbetween) seems less polished than the buttons appearing left to right. If there's no reason not to, I'd consider rearranging the buttons so that the ones appearing first are on the left.

I planted some tomatoes (do I need to do that manually?), and there are 'water' symbols nearby. I'm guessing they need water? No real question here, just a point where I'm not very clear on what needs to happen. I have a farmer dude.
    -- I just scrolled through the encyclopedia to read on tomatoes and tomato seeds. I would recommend a table of contents / index page when the encyclopedia is opened up, or some method to skip from the first entry to 'tomato' without tons of clicks.

In quest rewards, it's not clear that I'm getting blueprints instead of buildings. Maybe add a background sprite to blueprints to indicate that?

Under the forge build menu, I would like to see how many I have of each object. The current 'quantity' textbox I think is for building multiple objects, but it would be great to see if, say, I don't have any microphones and need to build some. (Or, conversely, that I already have one and don't need to worry about it.)

I think the "middle click to display building icons, click on icon to display building information" is a bit convoluted for an action. Maybe double clicking on the building could bring it up?

More tomato thoughts - a day later, they are still showing the water symbol. I have a farmer, and they are planted near a water pump with water available (blue meter is not empty). Not sure what to do there.

In the encyclopedia, the "building level" flag (showing the, say, fifth level walls) is maintained when the page is turned to a different building. Nitpick, but that was a little unexpected.

The second quest (collect resources) requires a certain amount of aquamarines. Are these mined from the same crystal pits that the crystal orbs come from?

My nutrient storage barrel doesn't seem to be storing any food. The barrel is empty, even though my inventory shows 14 tomatoes. Screenshot at https://imgur.com/a/d0x4DhM. My people are hungry!

All my citizens have now died of hunger. After the first one passed away, I set one of the remaining two's profession to farmer, maxed out farming on the preference scale, and planted three more tomato plants. I'm unsure if any additional farming was done.
    -- The game is continuing without any citizens right now, instead of giving a Game Over as expected. The top-lefft UI also says that I have one citizen left, although my character finder and priority menus don't show any.
    -- Additional note - I kept the default farmer's default preferences through the game. When I needed to change someone to an entertainer for the mission, I used my builder.

During my first game, I was able to get to 5 / 6 citizens, but never had more than 3 this time around. I'm not sure how to get more citizens.