That was happening with a mac build (which I have since taken down because we couldn't debug it). Are you experiencing that on Windows? If so, which version (10?7?) do you have a discrete graphics card?
Viewing post in After Hours: A Reverse Metroidvania comments
You should see the following structure (as I do on my machine when I unzip everything):
LD43_Data
--> a couple directories and a bunch of files
Mono
--> two directories
LD43.exe (run this one)
UnityCrashHandler64.exe
UnityPlayer.dll
Which directory was empty for you? Are you using the itch.io launcher, or just unzipping manually?
(Thank you for helping debug this!)
It's strange that you have an Empty AfterHours folder. Can you try running the game through the itch app?
https://itch.io/app