I just learned how to use a bit of blender and sprytile, I don't know if it might be even posible but I was wondering if you can switch between tilemaps on an object in the timeline, to make eyes blink or make mouth movements for example.
There isn't a built in way to do this with Sprytile, but it is possible to do this. How you approach it would depend on where you intend to use the asset.
If it's for sketchfab or a blender render the easiest way would be to create multiple tiles with the animation frames, and then use bone animations to hide the unwanted frames somewhere in the model that is hidden from view.
If it's for a game model, the most efficient way to do this is to animate the UVs in the engine. This would require some extra setup in separating the animated parts of the mesh and creating the system that animates the UVs in the engine which is a bit out of the scope for a forum post.
you can use the Blender addon Animall to make simple animations like water flow. There are a couple youtube tutorials online for simple animations, although this method requires quite some tweaking in the graph editor to set it to "linear".
you could also set the tweening to "constant" instead of "linear" and make it sudden, for stuff like blinking lights or opening/closing mouths.