My student keeps getting this error message when he tries playing his game. He has 8 games already published with no problems till this one
we have tried publishing it 3 times now
.
Probably you get the same error if you run the WebGL build locally (with a local web server), in the same browser. Did you try that?
It looks like an error that has nothing to do with itch.io. It seems to be something with audio automation. I advise to look into everything that you're doing related to audio parameters (mixer parameters, AudioSource parameters, ...)