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If you were granted commercial rights, and the person granting them had created the assets, you don't have a problem.  Do you have a readme file in the download or do you remember the artist's name?  You could see if he has other assets on itch.io and get his contact details from there.

If you're concerned that they were deleted due to copyright infringement i.e. the person uploading them had no rights to distribute them - you'd have to contact itch.io support with the details to see if they can help you - but you would need to know the name / package name or something that would help them identify it.

You could also do a reverse lookup:  https://support.google.com/websearch/answer/1325808?co=GENIE.Platform%3DDesktop&...

This will let you know if there is a source to the images you're using or where else they can be found on the internet.

Thank you for your reply Evolutuonary Games. The name of the user was Orlando Herrera and he seemed to delete all his art from his page. I know the art was all made by him and he gave permission in the description, just didn’t know if there was an issue since the art was free. I emailed him, but it seems he takes awhile to reply. I thought someone may have paid him to take them down so they can access them exclusively or he is using them in a game he is creating. Once again thank you for your reply.

I don't think you'll have a problem using those assets.   His social media pages still point to itch.io and that would indicate that you got legitimate assets with legitimate usage rights.