No, but if that's required then I'd highly suggest contacting itch for more upload space and packaging a single .zip file for the convenience of the majority of users. I can imagine that would otherwise be a deterrent to trying out your game.
Yeah, I am currently trying to convince them of it. If you ever make a game yourself, I highly suggest not to use paragon Assets if you're planning on sharing the vertical slice
If you are interested, the game has now been updated with a smaller and single file