Nope, you can use them without any of the RPG Maker licenses. :>
Viewing post in PIXEL FANTASY TILES - RMMZ RTP comments
Doesn't that create an issue with the Reid character? I don't imagine his character design is included with the MV license. ^^
Another question I almost forgot to ask! The iconset that comes packaged in here seems identical to an iconset posted under a "free but not for commercial projects" license. I don't see a similar stipulation here, so I'm wondering why that isn't clarified.