Hello, i want to ask something, how do I separate all this assets in different images.
Hello Mateo :),
Are you using a software to make your game ? Unity, Godot, RPG maker, ... ? Most of them will provide tools to use such atlas image.
If not (in case, for example, you're making your game from scratch for example), you'll need to split the atlas file into 16x16 squares with your programming language.