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I'm not 100% sure on how to interpret  "loading assets repeatedly", but I think u are asking about if I'm adding/removing new objects/elements in the DOM from time to time during the game's lifetime. If that is the case, then yes.

I mean every frame, not from time to time. I've seen beginners literally loading a sprite from disk anew every single frame.

I'm sorry, I don't know how to reply. I don't understand what " loading a sprite from disk anew every single frame" actually means. Is there an example that I can see somewhere ?

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You might want to get a more experienced developer look at your code.

Thanks for your advice and for trying to help me. I will do that if I find someone. If it's ok I do have one last question. What is the max size of the project that I can upload to Itch if I decide to go with "Downloadable" option?

(+1)

The limit is 1GB by default, two if you use Butler. You can also contact support and ask for an increase if that's not enough.