Hi
Depends what you mean. Is the 32x32 the base size or is it scaled up from a 16x16 base? If it's scaled then there's no issue, a 200% scale export option is included (because RPGMaker VX version uses 200%, so it's supported).
If 32x32 is your base size then it's a question of collision-matching and relative-sizing (to doorways, props and such), rather than being about the pixel scale. They MIGHT look just fine, or they might not, at that point it really depends on the specifics of the tilesets.
Thanks!