The gameplay is good and the art is well done. The song also matches very well and I did find myself interested of the story of the last ant, the Queen and Billy.
The main things I'd love to see improved would be removing the tutorial at the start of the second run (Even my first time through I thought it was a little overly long). I'd also love if the timer was bigger. I found myself enjoying the gameplay so much I'd forget the timer was an aspect and then die unceremoniously...
The itch page is decorated very nicely as well!