There are a lot of people that exist. But if you tell a story, you do not include all sorts of variations, just becaue they exist. Trans people exist too. So do children, disabled people, body builders and chain smokers.
So at which point would trans muscular smoking baby Mario in a wheelchair be a bit of a distraction in a jump & run game, that never actually adresses any of the highlighted qualities? Surely it must be a sign, if someone get's distracted by this and just plays another game.
My point is, that if you have gay Mario do the jumping, you better involve the gayness in the story in a satisfying manner. Else it is just a distraction. An informed quality.
There is nothing wrong with having gay Mario. Or muscular wheelchar Mario. This is a story decision of the writer. It is not a random chance roll of the dice what the story is about or what qualities the characters have, out of a pool of existing qualities.
If Mario would jump in the 1950s, he would have probaby had a cigarette in his mouth and depicting him as a non smoker would have been the distraction. In the 1890s it would have been a tobacco pipe, especially if he were to solve crimes. This "distraction" is about information value. The expected thing is the thing with the least information value when it happens. A stereotypical plumber from Italy having a mustache and being hetero is not news.
Presenting him with a full beard or as gay, is news. It subverts the trope. Being done good it is an interesting story. Though after glancing at tags, there seems to be a certain lack of gay jumper heroes. 170000k platformers. And maybe 500 gay/lgbt https://itch.io/games/genre-platformer/tag-gay https://itch.io/games/genre-platformer/tag-lgbt But I assume there is also a certain lack of hetero platformer heroes, since that quality is of no concern for jumping. Or as I phrase it, a distraction.