The game looks and sounds good and it follows the theme of the jam but it being so unforgiving and strict will probably only hurt it in the long run. If the game is going to end at any mistake it would probably be better to not immediately make it difficult because after a few attempts and only getting maybe 20 notes in I just gave up and moved on to the other games and I imagine others would give it far less time.
There is a reason why these types of games allow you to make multiple mistakes or have a scoring system.