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Hm ok, I'm not sure how fussy intersect it with tilesheets in that manner but it's good knowing it can scale up without an issue

As long as you scale in whole numbers (x2, x3, x4 or rather 200%, 300%, 400%) then your pixel art will always look good. So any multiple of 16 will work for this asset: 16x16, 32x32, 48x48, etc. If you try to mak,e it 24x24, then you'll ruin it 🤷‍♀️

One last thing, since I'm guessing you're using RPG Maker? Don't expect the autoiling to work. RPG Maker's autotiles are half the size of the regular tiles, mine are consistent. You'll need to place all your autotiles individually here.

No I'm  using Intersect, which just happens to work with VX ace style tilesheet sizes