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Thanks for letting me know. Can you please elaborate on how it is broken?  Do you get any error messages or is this the only thing appearing on the screen?

The program runs without any errors, and a seed generates. I thought it was caused by me adding another sprite and enemy, but extracting a whole new, unedited source code folder and running has the same issue.

I tried to run it on a new PC and I got the same error. It is caused by the newest version of Pygame, it seems. Try downgrading to Pygame 2.1.3 and it should work - at least it did for me :-)