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No, each tile is 16x16. 50x50 would be very high definintion for pixel art.

16x16 what?

How many pixels each tile is?

When I load the spritesheet in the game engine, writing 16x16 pixels gives me nothing.

If I write 50x50 pixels I can see the sprite

The tile is 16x16...meaning its only 16x16 pixels. You're not using your art program correctly.

Get a program like Asperite.