Great game you can connect some NES like controller, map buttons and play it. It would be interesting if there is an actually physical port for the NES because it fits and should be able to run on the NES.
Viewing post in Five Nights at Freddy's NES comments
If you have a controller hooked up to your pc, you can play with it!
But I don't think, that it could run on an nes because the code might be to suvvisticated, but mostly because of the static. The static would probably fry the nes's 2kb ram. But who knows, maybe a fnaf/nes enthusiast might attempt such a thing...
EDIT: I've checked, this game uses up to 120MB of RAM!