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(+1)

Do you have FFMPEG installed on your computer? GIFsquid might be trying to use the one on your system instead of the one included with it. You could try upgrading FFMPEG. Do me a favor and send me your email address in a PM, I want to send you some stuff.

(+1)

I think upgrading FFMPEG in GIFsquid\bin has resolved the issue; I'm generating large gifs with Turbo set to false with no issues. Thanks a lot for the troubleshooting help!