Not sure if you're aware of this, but when you try to launch the game on Windows 10 it throws a Windows Smart Screen error and tries to prevent you from launching the game. I've done a bit of research after encountering this error before, and it's apparently a problem with Unity games that have a custom icon. For some reason Windows flags the icon as a security risk.
EDIT: This Unity forum post describes the issue. https://forum.unity.com/threads/unity-5-the-digital-signature-is-not-valid-when-...