Aha. I haven't played Opportunity yet, so I wasn't aware that she was in both.
The main part that had me confused was her disappointed response to Diogenes's mention that 'cross-species insemination is all but unheard of,' considering that as yet no trans women have had the capacity to get pregnant anyway, though maybe that's explained in Opportunity
That said, I do think she mostly reads as trans even without that, but I don't think I could tell you why.