Funny character art. Good use of DDOLBANG's art for the dialogue sections!
I like the idea of a roguelike where you can select the aid of hololive talents to gain powerups. The powerups were pretty good and the various missions to unlock them kept the gameplay fresh.
That blue spinny thing enemy kept killing me in all my tries. They just went wild and killed me. I made it to the 6th room.
Edit: I figured out what was going on. It seems enemies go crazy fast once time runs out so I changed my strat to kill these fellows before the others and managed to survive until the end.

Some improvement suggestions:
- Maybe I didn't play enough or didn't get lucky in my runs but I didn't see a powerup to improve the attack power, cooldown or range. Most looked like they were going for heals. Edit: I found an attack related powerup in my next run after I wrote this comment.
- Using the trident to attack needs some i-frames as it moves you close to the target enemy and can end up in the player taking damage when trying to attack.
- It was funny that the player can still move after death. XD
- Please consider having the game's default volume to something closer to 50%. The default volume is very loud.
- It would also be nice if the volume settings were accessible from the title screen.
- I got a null reference exception when I spawned a tentacle thingy under the blue enemy and that froze the game.
Good job!