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i already reach form 23 for 682, i dont know how much evolution there is since the game doesnt tell me, so i dont know what's the final evolution is, and too afraid to use trascend since my progress already went too far

Then you're nowhere near the end. 682 has 32 Evolutions total, the last one is reached at 100T Cells. Transcention makes those numbers way easier to reach.

if my ask, whats the best amount of trascend use? i already use twice for triple income.

but start again from beginning again to trascend again seems hard 

Just keep Transcending. Maximise your Cell-growth via all Proteins, Tissues and not more than 7 Neurons.

If you only focus on Transcending, you'll reach 1Q Cells at around +2.000% Growth.