The audio tones sound great! I liked the colors lighting up also. It reminded me a little of the Milton Bradley game Simon, which I enjoyed!
I was confused about how to play at first. One idea I had was if the player plays the song forwards, tell them that they should play it backwards. It took me reading the itch page to understand, and even then I wasn't sure exactly what I needed to do until after a few moments it finally hit me. I like the idea and it was challenging to play the songs backwards. After I got the hang of it, it was over before I knew it... So maybe it could have been a little longer?
Great work fitting this challenging and interesting game into 64X64!