No, when you disable sales it says that you cannot sell more but the downloads are still accessible by people who have purchased. If the files are gone then itch has got rid of them. Thing is, they've never told me that is so
Viewing post in Game Update - Voluntary removal comments
It could be because I removed payment processors from my account.
The issue is that itch said any games removed permanently will trigger refunds for all purchasers. With the best will in the world, I couldn't risk them suddenly dropping $5k of refunds on me, so I disconnected paypal.
I'd recommend joining our Discord and I can sort out access without itch.