What should the Pixels Per Unit be set to in Unity?16 or 32？
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Depends on how big you want the player, for one unit(meter) use 32, for two use 16, or for half a unit use 64, if you want it any other size you divide 32 / the size you want. Another tip is to set the filter mode to point and the format to RGB 32 bit, these are the settings I use for crisp colors and realistic size.