Normally it would, but I'll grant an exception for this since it's for education. :)
Viewing post in Mystic Woods - 16x16 Pixel Art Asset Pack comments
It would be cool if you could add the following to your license: "Educators working for a publicly funded school may redistribute these assets to their students for non-commercial, educational purposes."
Probably not required since using your assets is likely allowed under Fair Dealings / Fair Use, but most students in public schools will not be able to access your assets through itch.io due to domain blocking for "games", and many teachers will just choose to use CC0 assets instead.