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16 pixels by 16 pixels is a common resolution for assets matching a low-res pixel art aesthetic. Although the assets may not be specifically 16x16, they are designed to work in a graphical setting where objects are around that size.

Set in a fictional universe, often (but not always) with fantastic or supernatural elements, alternate worlds, magic and monsters.

For that old school look and feel.

Related to the process of arranging game maps using a set of tile graphics (often call tilesets)