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You can kill the abyss boss once per week or you can force it to respawn by using "The World" card.
After sister has taken two doses you can continue to use them on yourself for a power/max hp boost.

She is located in the forest of ruin, accessed by Adventurers grove 2 (right, right, up) there is a sign telling you there's nothing there, you can walk behind the sign into the trees. There is a large hole in the ground, holding the charge attack with feather weapon you can fly over the hole to reach the other side.

1. When affection (red heart) and lust (purple heart) are high enough she will agree to wash your front

1. Cola is sold by the vending machine occasionally.
2. When Affection (red heart) and lust (purple heart) are high enough asking her to wash your front will trigger the second scene after she washes your arm.

According to the guide the effect is not yet implemente

Items out of bounds can be reached by equipping the Manekineko or using the vacuum weapon.