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'persistent' file is exist in 2 folders

1) %appdata%/renpy/lustfuldesire...

2) in your game folder, there is a folder name 'game' in this folder has a folder name 'save' and this save folder has another one persistent file 

a file name 'persistent' must be exist in those two folders among all of your save files. You must understand that persistent file ≠ save file and persistent file is a very important file. If you lose this file, your game may occur some bug.