Congrats on making a submission, and being able to participate in the jam!
Like others mentioned, I think it wasn't totally obvious what to exactly do to start the game, definitely worth adding something to the itch.io page on how to build and run the game for different platforms if possible (and which files to specifically open).
I tried using NVDA on windows, and didn't get any kind of audio affordance for the instructions. I think if these were in itch.io, that would be a good substitute, or conversely, just include an audio recording of the instructions at the start of the game! I think testing and making sure that the game is easy to run and launch with a screen reader (VoiceOver on Mac, or NVDA on Windows) would be extremely valuable.
All of that said, once I was able to run it, I understood and was able to find my way through the room. I was able to complete it in just under 2 minutes, and navigating around wasn't challenging at all.
Congrats again on building and sharing this for the jam.