No worries! The lot challenge will add a 50% nightly chance of a vampire invasion / night-time bite event no matter what world the lot is in, yeah. But you do need to add the challenge, otherwise there will be a zero percent chance of invasions in newer worlds.
The increased vampire aggression during walkbys will work in any world where vampire walkbys are a thing, which should be all of them except for Sixam, the career worlds, and the vacation worlds. This includes the newer ones.