I just want to say that this is probably the best trans rep I've ever seen in a game, and it's not even the focus of the game, which I honestly think is part of what makes it so good. In games that are specifically about a trans character, no matter how well it's handled, the character being trans has somewhat of a spotlight on it, and it ends up feeling a bit... "forced" isn't quite the right word, but it's the best I can come up with right now. Meanwhile, this game is about a character that happens to be trans, making the moments that it's brought up feel much more natural. It's not overly focused on. It's no different than Liam mentioning that he's Mexican. That's in addition to how organically these moments are handled in the game. Sam being trans isn't brought up more than it needs to be, and when it is mentioned, it always makes narrative sense. I also like that it touches on one of the more difficult parts of being trans, dysphoria, with Sam mentioning not wanting to buy clothes for herself because of her body and being nervous about Zombie Girl seeing her naked. I've played several games with trans MCs, and Sam definitely feels the most organic of all of them.
Another thing I really liked that is part of the focus of the game (SPOILERS FOR ANYONE READING THIS) is how when you finish Nicole's route and then replay it, you actually get to see some of the scenes from her perspective and see some of struggles she was going through from hiding who she was from Sam. That nightmare at the end of Chapter 4 actually made me cry, and it's really rare for things like movies and games to make me cry. I also like that the game actually acknowledges it if you name her Nicole, with Nicole's small reaction to the name, and Sam reacting to Nicole's name reveal at the end (and I like the small acknowledgement of using the default name, though I do wonder what's going on in Liam's head when he hears it, since he doesn't say anything about it).